Enjin Anders, Rabe Nadine, Nakanishi Stan T, Vallstedt Anna, Gezelius Henrik, Memic Fatima, Lind Magnus, Hjalt Tord, Tourtellotte Warren G, Bruder Carl, Eichele Gregor, Whelan Patrick J, Kullander Klas
Department of Neuroscience, Uppsala University, 751 23 Uppsala, Sweden.
J Comp Neurol. 2010 Jun 15;518(12):2284-304. doi: 10.1002/cne.22332.
Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems and are affected in spinal cord injury and in diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy. Using two screening approaches and in situ hybridization, we identified 159 genes expressed in typical cholinergic patterns in the spinal cord. These include two general cholinergic neuron markers, one gene exclusively expressed in motor neurons, and nine genes expressed in unknown subtypes of somatic motor neurons. Further, we present evidence that chondrolectin (Chodl) is expressed by fast motor neurons and that estrogen-related receptor beta (ERRbeta) is a candidate marker for slow motor neurons. In addition, we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells.
脊髓胆碱能神经元在自主神经系统和躯体神经系统的运动功能中起着关键作用，并且在脊髓损伤以及诸如肌萎缩侧索硬化症（ALS）和脊髓性肌萎缩症等疾病中会受到影响。利用两种筛选方法和原位杂交技术，我们鉴定出159个在脊髓中以典型胆碱能模式表达的基因。其中包括两种一般胆碱能神经元标志物、一个仅在运动神经元中表达的基因，以及九个在躯体运动神经元未知亚型中表达的基因。此外，我们提供的证据表明软骨凝集素（Chodl）由快运动神经元表达，而雌激素相关受体β（ERRbeta）是慢运动神经元的候选标志物。另外，我们建议将配对样同源结构域转录因子2（Pitx2）作为胆碱能分隔细胞的标志物。
