泰国白米、红米和黑米麸皮提取物中总酚含量及其抗氧化活性的研究。

Study on total phenolic contents and their antioxidant activities of Thai white, red and black rice bran extracts.

作者信息

Muntana N, Prasong S

机构信息

The Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand.

出版信息

Pak J Biol Sci. 2010 Feb 15;13(4):170-4. doi: 10.3923/pjbs.2010.170.174.

DOI:10.3923/pjbs.2010.170.174

PMID:20437683

Abstract

In this study, different brans of Thai rice cultivars which were divided into 3 groups: white color (Hom mali 105, Supan, Saohai, Hom chaiya and Hom jun), red color (3256, GS 18003, 21606, 21699 and 5718) and black color (96041-20, 96051-37, 96004-49, 96023-35 and 96065-42) were investigated their Total Phenolic Content (TPC) and antioxidant activities. The rice brans were firstly extracted using methanol. The extracts were analyzed using Folin-Ciocalteau method for total phenolic content while thiocyanate method and 1,1-diphenyl-2-picrylhydrasyl (DPPH) free radical-scavenging assay were used for antioxidant activities determination. The results indicated that the total phenolic content of white, red and black rice bran extract were in the range of 0.8931-0.9884, 1.0103-1.0494 and 1.0810-1.2239 mg gallic acid equivalent (GAE mg(-1)), respectively. With thiocyanate method, percentage inhibition were in the range of 10.15-20.68, 30.64-38.80 and 25.52-26.28 for white, red and black rice bran extract, respectively. With DPPH radical-scavenging assay, methanolic extract of 5718 showed the highest (IC50=0.0057 mg mL(-1)) while Homchaiya showed the lowest (IC50=0.2582 mg mL(-1)) activities. All of extracts showed lower activity than BHA (IC50=0.0012 mg mL(-1)). However, the antioxidant activity of all rice bran extracts indicated high antioxidant efficiency in the following order: red>black>white color rice brans. It is a promising that Thai rice bran are potential antioxidant sources.

摘要

在本研究中，将泰国水稻品种的不同谷 bran 分为 3 组：白色（Hom mali 105、Supan、Saohai、Hom chaiya 和 Hom jun）、红色（3256、GS 18003、21606、21699 和 5718）和黑色（96041 - 20、96051 - 37、96004 - 49、96023 - 35 和 96065 - 42），对其总酚含量（TPC）和抗氧化活性进行了研究。首先用甲醇提取水稻谷 bran。提取物采用福林 - 酚法分析总酚含量，同时采用硫氰酸盐法和 1,1 - 二苯基 - 2 - 苦基肼（DPPH）自由基清除试验测定抗氧化活性。结果表明，白色、红色和黑色水稻谷 bran 提取物的总酚含量分别在 0.8931 - 0.9884、1.0103 - 1.0494 和 1.0810 - 1.2239 mg 没食子酸当量（GAE mg(-1)）范围内。采用硫氰酸盐法时，白色、红色和黑色水稻谷 bran 提取物的抑制率分别在 10.15 - 20.68、30.64 - 38.80 和 25.52 - 26.28 范围内。采用 DPPH 自由基清除试验时，5718 的甲醇提取物活性最高（IC50 = 0.0057 mg mL(-1)），而 Homchaiya 的活性最低（IC50 = 0.2582 mg mL(-1)）。所有提取物的活性均低于 BHA（IC50 = 0.0012 mg mL(-1)）。然而，所有水稻谷 bran 提取物的抗氧化活性按以下顺序显示出高抗氧化效率：红色 > 黑色 > 白色水稻谷 bran。泰国水稻谷 bran 有潜力成为抗氧化剂来源，这是很有前景的。