National Centre of Excellence in Molecular Biology, University of the Punjab Lahore, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan.
Infect Genet Evol. 2010 Jul;10(5):595-600. doi: 10.1016/j.meegid.2010.04.012. Epub 2010 May 9.
Information regarding the changing pattern in hepatitis C virus (HCV) genotypes/subtypes and resulting disease outcome is not well known. The specific objective of this study was to find out the frequency distribution of HCV genotypes and changing pattern of various HCV genotypes overtime in well-characterized Pakistani HCV isolates. The genotype distribution of HCV from all the four provinces of Pakistan was tracked for a period of 10 years (2000-2009) on total 20,552 consecutive anti-HCV and HCV RNA positive patients sample using type-specific genotyping assay. Of these, 16,891 (82.2%) samples were successfully genotyped. Of these 11,189 (54.4%) were males and 9363 (45.55%) were females. Of the successfully genotyped samples, 12,537 (74.2%) were with 3a, 1834 (10.9%) with 3b, 50 (0.24%) with 3c, 678 (3.3%) with 1a, 170 (0.83%) with 1b, 49 (0.24%) with 1c, 431 (2.1%) with 2a, 48 (0.23%) with 2b, 3 (0.01%) with 2c, 13 (0.06%) with 5a, 12 (0.06%) with 6a, 101 (0.49%) with 4, and 965 (4.7%) were with mixed-genotype infection. A changing pattern of HCV genotypes prevalence was observed in Pakistan overtime, with an increase in the relative proportion of genotype 3a and mixed genotypes and a decrease of genotypes 3b, 2b, 4, 5a and 2a. This changed HCV genotype pattern might have direct impact on HCV disease outcome and new therapeutic strategies may be needed.
有关丙型肝炎病毒（HCV）基因型/亚型变化模式及由此导致的疾病结局的信息尚不清楚。本研究的具体目的是在经过充分特征鉴定的巴基斯坦 HCV 分离株中，确定 HCV 基因型的频率分布和随时间推移各种 HCV 基因型的变化模式。在 10 年（2000-2009 年）期间，对来自巴基斯坦四个省的 HCV 基因型分布进行了跟踪，共对 20552 例连续抗 HCV 和 HCV RNA 阳性患者样本进行了基于型特异性基因分型的检测。其中，16891 例（82.2%）样本成功进行了基因分型。其中 11189 例（54.4%）为男性，9363 例（45.55%）为女性。在成功进行基因分型的样本中，12537 例（74.2%）为 3a 型，1834 例（10.9%）为 3b 型，50 例（0.24%）为 3c 型，678 例（3.3%）为 1a 型，170 例（0.83%）为 1b 型，49 例（0.24%）为 1c 型，431 例（2.1%）为 2a 型，48 例（0.23%）为 2b 型，3 例（0.01%）为 2c 型，13 例（0.06%）为 5a 型，12 例（0.06%）为 6a 型，101 例（0.49%）为 4 型，965 例（4.7%）为混合基因型感染。在巴基斯坦，HCV 基因型的流行率随时间发生了变化，3a 基因型和混合基因型的相对比例增加，而 3b、2b、4、5a 和 2a 基因型的比例减少。这种 HCV 基因型模式的变化可能对 HCV 疾病结局产生直接影响，可能需要新的治疗策略。
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