Horikawa K, Sera N, Otofuji T, Murakami K, Tokiwa H, Iwagawa M, Izumi K, Otsuka H
Department of Health Science, Fukuoka Environmental Research Center, Japan.
Carcinogenesis. 1991 Jun;12(6):1003-7. doi: 10.1093/carcin/12.6.1003.
3,9- and 3,7-Dinitrofluoranthene (3,9- and 3,7-DNF), 3-nitrofluoranthene (3-NF) and benzo[a]pyrene (B[a]P) were tested for pulmonary carcinogenicity by intrapulmonary implantation of the compounds into rat lung. These chemicals were given in various doses as suspensions in beeswax-trycaprylin and the animals were observed for 100 weeks. The control group received no drugs. The incidences of lung tumors were 19/21 (90.5%), 7/10 (70%) and 1/10 (10%) in rats treated with 200, 100 and 50 micrograms of 3,9-DNF, 4/9 (44.4%), 3/10 (30%) and 0/10 (0%) in rats treated with 200, 100 and 50 micrograms of B[a]P, 12/22 (54.5%) in rats treated with 200 micrograms of 3,7-DNF and 1/20 (5%) in rats treated with 1000 micrograms of 3-NF respectively. No lung tumors were found in control rats. The incidence of lung tumors induced by 3,9-DNF was twice as high as that induced by B[a]P, when the equivalent dose levels of the two compounds were compared. Histologically, most of the tumors induced by 3,9- and 3,7-DNF and B[a]P were squamous cell carcinomas.
通过将3,9 - 二硝基荧蒽（3,9 - DNF）、3,7 - 二硝基荧蒽（3,7 - DNF）、3 - 硝基荧蒽（3 - NF）和苯并[a]芘（B[a]P）经肺内植入大鼠肺部来测试其肺致癌性。这些化学物质以不同剂量悬浮于蜂蜡 - 三辛酸甘油酯中给药，并对动物观察100周。对照组未给药。用200、100和50微克3,9 - DNF处理的大鼠肺肿瘤发生率分别为19/21（90.5%）、7/10（70%）和1/10（10%）；用200、100和50微克B[a]P处理的大鼠肺肿瘤发生率分别为4/9（44.4%）、3/10（30%）和0/10（0%）；用200微克3,7 - DNF处理的大鼠肺肿瘤发生率为12/22（54.5%），用1000微克3 - NF处理的大鼠肺肿瘤发生率为1/20（5%）。对照组大鼠未发现肺肿瘤。当比较两种化合物的等效剂量水平时，3,9 - DNF诱导的肺肿瘤发生率是B[a]P诱导的两倍。组织学上，3,9 - 和3,7 - DNF以及B[a]P诱导的大多数肿瘤为鳞状细胞癌。
