Du Zhuo, Yu Yong-Liang, Wang Jian-Hua
Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110004, China.
Macromol Biosci. 2009 Jan 9;9(1):55-62. doi: 10.1002/mabi.200800200.
A micro-column packed with PDDA-wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA-MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 microL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6-120 microg, resulting in an enrichment factor of 14. A sampling frequency of 15 h(-1) is achieved, along with a precision of 4.5% at 25 microg x mL(-1) BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.
在顺序注射系统中，填充有聚二烯丙基二甲基氯化铵包裹的多壁碳纳米管的微柱有助于酸性蛋白质物种的选择性吸附。吸附在聚二烯丙基二甲基氯化铵-多壁碳纳米管复合材料上的蛋白质随后用柠檬酸盐缓冲液作为洗脱剂进行洗脱收集。进样体积为2.0 mL，洗脱剂体积为200 μL时，在6-120 μg范围内牛血清白蛋白的保留效率为100%，回收率为90%，富集因子为14。采样频率达到15 h⁻¹，在25 μg·mL⁻¹牛血清白蛋白时精密度为4.5%。通过处理人全血以连续分离酸性蛋白质，证明了该系统的实际适用性。
