College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Proteomics. 2010 Jul;10(13):2471-85. doi: 10.1002/pmic.200900763.
Scorpion venoms contain a vast untapped reservoir of natural products, which have the potential for medicinal value in drug discovery. In this study, toxin components from the scorpion Heterometrus petersii venom were evaluated by transcriptome and proteome analysis.Ten known families of venom peptides and proteins were identified, which include: two families of potassium channel toxins, four families of antimicrobial and cytolytic peptides,and one family from each of the calcium channel toxins, La1-like peptides, phospholipase A2,and the serine proteases. In addition, we also identified 12 atypical families, which include the acid phosphatases, diuretic peptides, and ten orphan families. From the data presented here, the extreme diversity and convergence of toxic components in scorpion venom was uncovered. Our work demonstrates the power of combining transcriptomic and proteomic approaches in the study of animal venoms.
蝎毒液中蕴藏着巨大的未开发的天然产物资源库，具有在药物发现中具有药用价值的潜力。在这项研究中，通过转录组和蛋白质组分析评估了来自 Heterometrus petersii 蝎毒液的毒素成分。鉴定出十种已知的毒液肽和蛋白质家族，包括：两种钾通道毒素家族，四种抗菌和细胞溶解肽家族，以及钙通道毒素、La1 样肽、磷脂酶 A2 各一种家族和丝氨酸蛋白酶。此外，我们还鉴定出 12 种非典型家族，包括酸性磷酸酶、利尿剂肽和十个孤儿家族。从这里呈现的数据中，揭示了蝎毒液中有毒成分的极端多样性和趋同进化。我们的工作证明了在动物毒液研究中结合转录组学和蛋白质组学方法的强大功能。
