Lavilla Isela, Rodríguez-Liñares Genma, Garrido Josefina, Bendicho Carlos
Departamento de Química Analítica y Alimentaria, Facultad de Química, Universidad de Vigo, As Lagoas-Marcosende s/n, 36310, Vigo, Spain.
J Environ Monit. 2010 Mar;12(3):724-30. doi: 10.1039/b920379f. Epub 2010 Jan 15.
The accumulation patterns of different trace elements (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) were studied in three species of dragonfly larvae (Cordulegaster boltoni, Boyeria irene and Onychogomphus uncatus). Additionally these species were assessed as potential biomonitors in a lotic ecosystem (Louro River, Spain). Element concentrations were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) in both dragonfly larvae and river sediments. The surface of the larvae was observed and analyzed with scanning electron microscopy (SEM). A desorption test was used to establish the percentage of element adsorbed into the exoskeleton. Field biomonitoring studies were made considering the first part of the river as a control area in relation to the second part, which was severely polluted by industrial and domestic activities. Upon application of principal component analysis (PCA), two different element groups were found in relation to element bioaccumulation. Cu, Cd and Zn were mainly associated with the inner part of the larvae. As, Cr, Fe, Mn, Ni and Zn were found on the outer body parts, related with deposition of oxyhydroxides in the hydrocycle. SEM revealed a layer of inorganic particles, similar in composition to fine bed sediments. Significant relations between the element content of this group and that of sediments at the sampling site were found. Differences in bioaccumulation for each of the three species, except for As, were observed. Dragonfly larvae revealed their ability to reflect environmental concentrations of As in freshwater media.
研究了三种蜻蜓幼虫（博氏大蜓、艾氏伟蜓和弯尾琵蟌）体内不同微量元素（砷、镉、铬、铜、铁、锰、镍、铅和锌）的积累模式。此外，还评估了这些物种作为流水生态系统（西班牙卢罗河）中潜在生物监测器的情况。通过电感耦合等离子体发射光谱法（ICP - OES）测定了蜻蜓幼虫和河流沉积物中的元素浓度。用扫描电子显微镜（SEM）观察和分析了幼虫的体表。采用解吸试验确定吸附在外骨骼上的元素百分比。进行了野外生物监测研究，将河流的第一部分作为对照区域，与受工业和家庭活动严重污染的第二部分进行对比。应用主成分分析（PCA）后，发现与元素生物积累相关的两个不同元素组。铜、镉和锌主要与幼虫内部相关。砷、铬、铁、锰、镍和锌存在于幼虫身体外部，与水圈中氢氧化物的沉积有关。扫描电子显微镜显示有一层无机颗粒，其成分与细河床沉积物相似。发现该组元素含量与采样点沉积物的元素含量之间存在显著关系。观察到三种物种中除砷外，每种物种的生物积累都存在差异。蜻蜓幼虫显示出它们能够反映淡水介质中砷的环境浓度。
