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[Chick embryo culture using duck egg shell--first successful hatch ].

作者信息

Miura K, Sueyoshi M, Jinbu M, Oka M

机构信息

National Institute of Animal Health, Ibaraki, Japan.

出版信息

Jikken Dobutsu. 1991 Apr;40(2):251-4. doi: 10.1538/expanim1978.40.2_251.

Abstract

The suitability of duck egg shell (DES) for chick embryo culture was investigated. Chick embryos were transferred into DESs with all egg contents after 3 days of normal incubation and cultured. The vessels made of polyethylene cling film were used for shell-less control. Among 35 embryos cultured in DESs, 21 survived until 16 days of incubation (13 days after transfer) and finally 3 newly hatched chicks were obtained at 22 days of incubation. One of them died 4 days later, but remaining two became full-grown cocks showing normal body weight and production of fertile sperms. Among 37 embryos cultured in polyethylene vessels, none survived over the period of 19 days of incubation. It is suggested that DES culture system may be useful for the various experiments using chick embryos.

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