Dunn B E, Clark N B, Scharf K E
J Exp Zool Suppl. 1987;1:33-7.
Chick embryos grown in shell-less culture are calcium-deficient by 9 days and retarded in growth by 13 days of incubation (Dunn and Boone, Poult. Sci., 56:662-672, 1977). To determine whether addition of exogenous calcium might stimulate growth and/or survival of cultured embryos, calcium supplementation was attempted. Calcium supplementation between days 11 and 17 resulted in significant increases in both total embryo and serum calcium. Addition of shell pieces oriented with shell membranes onto the chorioallantoic membrane (CAM) resulted in significant stimulation of calcium transport by the CAM. However, growth of supplemented embryos was not increased to the same degree as were embryonic serum and total calcium levels. It is concluded that at least one factor other than calcium deficiency is responsible for retarded growth of shell-less cultured embryos.
在无壳培养中生长的鸡胚到9日龄时缺钙，孵化至13日龄时生长迟缓（邓恩和布恩，《家禽科学》，56:662 - 672，1977）。为了确定添加外源钙是否可能刺激培养胚胎的生长和/或存活，尝试进行了钙补充。在第11天至17天补充钙导致胚胎总钙量和血清钙显著增加。将带有壳膜的壳碎片添加到尿囊绒膜（CAM）上导致CAM对钙转运的显著刺激。然而，补充钙的胚胎的生长并没有增加到与胚胎血清和总钙水平相同的程度。得出的结论是，除了缺钙之外，至少还有一个因素导致无壳培养胚胎生长迟缓。
