INSERM U1019, F-59000 Lille, France.
Vaccine. 2010 Jun 17;28(27):4340-7. doi: 10.1016/j.vaccine.2010.04.062. Epub 2010 May 4.
Heterologous prime-boost regimens are a valuable strategy to improve the generation of effector-memory T cell responses against intracellular pathogens. In this study we show that newborn mice vaccinated with bacillus Calmette-Guérin (BCG) and boosted with heparin-binding haemagglutinin (HBHA) had enhanced protective immunity against intranasal or aerosol Mycobacterium tuberculosis challenge over non-boosted mice, as evidenced by a considerable reduction of mycobacterial load in spleen and lung. The route of HBHA delivery had a differential impact on cytokine and antibody production in BCG-primed mice. The prime-boost regimen induced not only HBHA-specific IFN-gamma, but also other cytokines, such as IL-12 and TGF-beta, which may be associated with the generation of lung Th1 effector-memory lymphocytes, responsible for the enhanced protection against M. tuberculosis challenge.
异源初免-加强免疫方案是提高针对细胞内病原体的效应记忆 T 细胞反应的一种有效策略。在这项研究中，我们表明，用卡介苗（BCG）免疫接种并加强肝素结合血凝素（HBHA）的新生小鼠，在鼻腔内或气溶胶结核分枝杆菌攻击方面比未加强的小鼠具有更强的保护免疫力，这体现在脾脏和肺部的分枝杆菌负荷量显著减少。HBHA 给药途径对 BCG 初免小鼠的细胞因子和抗体产生有不同的影响。初免-加强免疫方案不仅诱导了 HBHA 特异性 IFN-γ，还诱导了其他细胞因子，如 IL-12 和 TGF-β，这可能与肺 Th1 效应记忆淋巴细胞的产生有关，而 Th1 效应记忆淋巴细胞负责增强对 M. tuberculosis 挑战的保护。
