Ohlendieck K, Campbell K P
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242.
FEBS Lett. 1991 Jun 3;283(2):230-4. doi: 10.1016/0014-5793(91)80595-t.
Dystrophin, which is absent in skeletal muscle of Duchenne muscular dystrophy patients, has not been considered to play a major structural role in the cell membrane of skeletal muscle because of its low abundance (approximately 0.002% of total muscle protein). Here, we have determined the relative abundance of dystrophin in a membrane cytoskeleton preparation and found that dystrophin constitutes approximately 5% of the total membrane cytoskeleton fraction of skeletal muscle sarcolemma. In addition, dystrophin can be removed from sarcolemma by alkaline treatment. Thus, our results have demonstrated that dystrophin is a major component of the subsarcolemmal cytoskeleton in skeletal muscle and suggest that dystrophin could play a major structural role in the cell membrane of skeletal muscle.
肌营养不良蛋白在杜氏肌营养不良症患者的骨骼肌中缺失，由于其丰度较低（约占肌肉总蛋白的0.002%），一直未被认为在骨骼肌细胞膜中起主要结构作用。在此，我们测定了膜细胞骨架制剂中肌营养不良蛋白的相对丰度，发现肌营养不良蛋白约占骨骼肌肌膜总膜细胞骨架组分的5%。此外，碱性处理可将肌营养不良蛋白从肌膜上去除。因此，我们的结果表明，肌营养不良蛋白是骨骼肌肌膜下细胞骨架的主要成分，并提示肌营养不良蛋白可能在骨骼肌细胞膜中起主要结构作用。
