Forschungszentrum Dresden-Rossendorf, Research Site Leipzig, Institute of Radiopharmacy, Permoserstr. 15, 04318 Leipzig, Germany.
J Chromatogr A. 2010 Jun 11;1217(24):3855-62. doi: 10.1016/j.chroma.2010.04.018. Epub 2010 May 4.
High-performance liquid chromatography enantioseparation of vesamicol and six novel azaspirovesamicols (amino alcohols) was accomplished on different chiral stationary phases (CSPs) by using an optical rotation based chiral detector for identification of the resolved enantiomers. The Pirkle-type column Reprosil Chiral-NR was found to be most suitable for chiral resolution in normal phase (NP) mode; all compounds could be enantioseparated successfully. Also the cellulose-based column Reprosil Chiral-OM showed appropriate separation properties by using NP conditions. The amylose-type column Reprosil Chiral-AM-RP was most suitable for enantioseparation in reversed phase (RP) mode; five out of seven compounds were resolved. This CSP showed a considerably higher capability for chiral recognition of vesamicol derivatives in RP mode than the corresponding cellulose-based column Reprosil Chiral-OM-RP. Enantioseparation with the teicoplanin aglycone-based column Reprosil Chiral-AA was successful under polar ionic mobile phase conditions.
高效液相色谱手性拆分瓦氏胺和六种新型氮杂螺环[4,5]癸烷（氨基醇），通过使用基于旋光的手性检测器来识别拆分的对映异构体，在不同的手性固定相（CSP）上完成。发现 Pirkle 型柱 Reprosil Chiral-NR 最适合正相（NP）模式下的手性拆分；所有化合物都能成功分离。同样，使用 NP 条件，基于纤维素的柱 Reprosil Chiral-OM 也表现出适当的分离性能。直链淀粉型柱 Reprosil Chiral-AM-RP 最适合反相（RP）模式下的对映体拆分；七种化合物中有五种得到拆分。与相应的基于纤维素的柱 Reprosil Chiral-OM-RP 相比，该 CSP 在 RP 模式下对瓦氏胺衍生物的手性识别具有更高的能力。在极性离子流动相条件下，使用 Teicoplanin 苷元衍生的柱 Reprosil Chiral-AA 也成功地进行了对映体拆分。
