Aslanidi K B, Chailakhyan L M, Kublik L N, Marachova I I, Potapova T V, Vinogradova T A
A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
FEBS Lett. 1991 Jun 3;283(2):295-7. doi: 10.1016/0014-5793(91)80612-7.
Low ouabain concentration (1 x 10(-6) M) is shown to decrease intracellular K+ (K+in) and to increase intracellular Na+ (Na+in) in human fibroblast cell cultures. The same ouabain concentration was without effect upon K+in ad Na+in in rodent cultures such as BHK-21, mouse fibroblasts and rat glyoma C6 cells. K+in and Na+in in the mixed cultures of human and BHK-21 fibroblasts or human and mouse fibroblasts were found to be resistant to 1 x 10(-6) M ouabain whereas that of the mixtures of human and rat glyoma C6 cells proved to be ouabain-sensitive. The gap-junction-mediated dye transfer was revealed between human and BHK-21 cells. Such an effect was very small in the human-C6 cell mixed culture. It is concluded that cells with active ion pumps can support the maintenance of K+ and Na+ gradients in cells with inactive pumps, provided that effective ion transport via gap junctions takes place.
低浓度哇巴因（1×10⁻⁶ M）可降低人成纤维细胞培养物中的细胞内钾离子（K⁺in）浓度，并升高细胞内钠离子（Na⁺in）浓度。相同浓度的哇巴因对啮齿类动物培养物，如BHK - 21细胞、小鼠成纤维细胞和大鼠胶质瘤C6细胞中的K⁺in和Na⁺in没有影响。在人和BHK - 21成纤维细胞或人和小鼠成纤维细胞的混合培养物中，K⁺in和Na⁺in对1×10⁻⁶ M哇巴因具有抗性，而人和大鼠胶质瘤C6细胞混合物中的K⁺in和Na⁺in则对哇巴因敏感。在人和BHK - 21细胞之间发现了间隙连接介导的染料转移。这种效应在人 - C6细胞混合培养物中非常小。得出的结论是，只要通过间隙连接发生有效的离子转运，具有活性离子泵的细胞就能支持无活性泵细胞中钾离子和钠离子梯度的维持。
