Harwood A J, Early A E, Jermyn K A, Williams J
Imperial Cancer Research Fund, Clare Hall Laboratory, PottersBar, Herts, UK.
Differentiation. 1991 Feb;46(1):7-13. doi: 10.1111/j.1432-0436.1991.tb00860.x.
We show that the anterior, prestalk region of the Dictyostelium slug contains cells which express, or have expressed, a prespore-specific marker. We term these cells "prespore-like cells" (PLC). In newly formed slugs there is a sharp prespore/prestalk boundary, with very few PLC, but after several days of migration the clear demarcation between prespore and prestalk zones breaks down because the number of PLC increases dramatically. This is consistent with previous observations showing there to be rapid interchange of cells between the prestalk and prespore regions. This is not, however, their only source, as a scattering of PLC appear when separate prestalk and prespore regions first become apparent at the time of tip formation. Also, at culmination, there is respecification of "prespore" cells at the prestalk/prespore boundary to form part of the mature stalk. The existence of these cells, and of PLC, may explain why we find prespore-specific mRNAs in mature stalk cells.
我们发现，盘基网柄菌蛞蝓的前柄细胞区域含有表达或曾经表达过前孢子特异性标记物的细胞。我们将这些细胞称为“类前孢子细胞”（PLC）。在新形成的蛞蝓中，前孢子/前柄界限分明，类前孢子细胞很少，但经过几天的迁移后，前孢子区和前柄区之间清晰的界限消失了，因为类前孢子细胞的数量急剧增加。这与之前的观察结果一致，即前柄区和前孢子区之间存在细胞的快速交换。然而，这并不是它们的唯一来源，因为在尖端形成时，当单独的前柄区和前孢子区首次出现时，就会出现一些分散的类前孢子细胞。此外，在发育成熟时，前柄/前孢子边界处的“前孢子”细胞会重新分化，形成成熟柄的一部分。这些细胞以及类前孢子细胞的存在，可能解释了为什么我们在成熟柄细胞中发现了前孢子特异性mRNA。
