大肠杆菌α-溶血素基因的检测及其在人粪便阴沟肠杆菌菌株中的表达。

Detection of Escherichia coli alpha-haemolysin genes and their expression in a human faecal strain of Enterobacter cloacae.

作者信息

Prada J, Beutin L

机构信息

Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, F.R.G.

出版信息

FEMS Microbiol Lett. 1991 Mar 15;63(1):111-4. doi: 10.1016/0378-1097(91)90537-k.

DOI:10.1016/0378-1097(91)90537-k

PMID:2044935

Abstract

Faecal samples of 200 infants were investigated for haemolytic Enterobacteriaceae. Forty infants were carrying alpha-haemolysin producing Escherichia coli, two carried haemolytic strains of Morganella morganii and one infant carried a haemolytic strain of Enterobacter cloacae. The M. morganii and E. cloacae strains were found to produce alpha-haemolysin which was tested with a specific monoclonal antibody and by DNA-hybridization with an alpha-haemolysin specific gene probe. To our knowledge this is the first report of alpha-haemolysin production found in a strain of E. cloacae.

摘要

对200名婴儿的粪便样本进行了溶血性肠杆菌科细菌的调查。40名婴儿携带产α-溶血素的大肠杆菌，2名婴儿携带摩根氏摩根菌的溶血菌株，1名婴儿携带阴沟肠杆菌的溶血菌株。发现摩根氏摩根菌和阴沟肠杆菌菌株产生α-溶血素，用特异性单克隆抗体进行检测，并通过与α-溶血素特异性基因探针进行DNA杂交检测。据我们所知，这是在阴沟肠杆菌菌株中发现产α-溶血素的首次报道。