Eberspächer B, Hugo F, Pohl M, Bhakdi S
Institute of Medical Microbiology, University of Giessen, West Germany.
J Med Microbiol. 1990 Nov;33(3):165-70. doi: 10.1099/00222615-33-3-165.
Haemolysin produced by a clinical isolate of Morganella morganii was examined for antigenic relatedness to the haemolysin of Escherichia coli and for similarities in mode of action. The M. morganii haemolysin migrated in SDS-PAGE as a single protein band with a slightly higher molecular weight than that of E. coli haemolysin. Several murine monoclonal antibodies against E. coli haemolysin cross-reacted with the M. morganii haemolysin in Western blots. Diminished haemolysis in the presence of osmotically-stabilising solutes indicated the formation of a pore by M. morganii haemolysin with an effective diameter of 1.5-3 nm. Results from dose-response experiments indicated that a single "hit" was sufficient for lysis of an erythrocyte. Detergent solubilisation of toxin-treated membranes led to recovery of bound toxin exclusively in monomeric form. M. morganii haemolysin was a potent leucocidin, that caused rapid leakage of ATP and death of human polymorphonuclear leucocytes. Under in-vitro conditions M. morganii haemolysin displayed similar leucocidal and haemolytic efficiency. The data demonstrate that M. morganii haemolysin shows functional properties virtually identical with those of E. coli haemolysin.
对摩根氏摩根菌临床分离株产生的溶血素进行了检测，以确定其与大肠杆菌溶血素的抗原相关性及其作用方式的相似性。摩根氏摩根菌溶血素在SDS-PAGE中迁移为单一蛋白条带，其分子量略高于大肠杆菌溶血素。几种抗大肠杆菌溶血素的鼠单克隆抗体在Western印迹中与摩根氏摩根菌溶血素发生交叉反应。在存在渗透稳定溶质的情况下溶血作用减弱，表明摩根氏摩根菌溶血素形成了有效直径为1.5 - 3 nm的孔。剂量反应实验结果表明，单次“打击”就足以使红细胞裂解。经毒素处理的膜用去污剂溶解后，仅以单体形式回收结合的毒素。摩根氏摩根菌溶血素是一种强效白细胞毒素，可导致ATP迅速泄漏和人多形核白细胞死亡。在体外条件下，摩根氏摩根菌溶血素表现出相似的杀白细胞和溶血效率。数据表明，摩根氏摩根菌溶血素显示出与大肠杆菌溶血素几乎相同的功能特性。
