Sharma S, Sandhu D K, Bagga P S
Microbiology Unit, School of Life Sciences, Guru Nanak Dev University, Amritsar, India.
FEMS Microbiol Lett. 1991 Mar 15;63(1):99-104. doi: 10.1016/0378-1097(91)90535-i.
Electrophoretic data revealed the presence of various isozymes of endoglucanase and beta-glucosidase, the number of which varied from one to three in various species of the genus Aspergillus. pH 5.0 was optimum for all the isozymes whereas metal ion treatment showed complete inhibition of almost all the isozymes by Hg2+ and partial inhibition by Ca2+ and Co2+ of isozymes of both the enzymes. An alteration in the electrophoretic mobility of isozymes of beta-glucosidase was also noticed in some species with Hg2+ treatment.
电泳数据显示存在内切葡聚糖酶和β-葡萄糖苷酶的多种同工酶，在曲霉属的不同物种中，其数量从一种到三种不等。pH 5.0对所有同工酶而言是最适宜的，而金属离子处理显示，Hg2+几乎完全抑制了所有同工酶，Ca2+和Co2+对这两种酶的同工酶有部分抑制作用。在一些物种中，用Hg2+处理后，还注意到β-葡萄糖苷酶同工酶的电泳迁移率发生了变化。
