Singh S, Brar J K, Sandhu D K, Kaur A
Department of Microbiology, Guru Nanak Dev University, Amritsar, India.
J Basic Microbiol. 1996;36(4):289-96. doi: 10.1002/jobm.3620360412.
Maximum cellulase production in Aspergillus terreus was obtained at a temperature of 28 degrees C, pH 4.0 and a substrate concentration of 1% CMC. Variability in cellulase enzyme production and isozyme polymorphism of endo-beta-1,4-glucanase and beta-1,4-glucosidase was studied in 45 natural isolates of A. terreus. Different electrophoretic patterns were evident for endoglucanase. Three zones of activity viz EG 1, EG 11 and EG 111 were observed showing different electrophoretic mobilities. Some of the isolates exhibited the presence of null alleles for EG 1. During development EG 1 and EG 11 were observed throughout while EG 111 appeared on the eighth day. For beta-1,4-glucosidase two zones of activity viz beta-glu 1 and beta-glu 11 were observed. beta-glu 1 showed variable electrophoretic mobilities. beta-glu 1 appeared throughout during development while beta-glu 11 appeared on the twelfth day.
在曲霉中，当温度为28摄氏度、pH值为4.0且底物浓度为1%的羧甲基纤维素（CMC）时，纤维素酶产量达到最高。对45株土曲霉的自然分离株进行了纤维素酶产量变异性以及内切-β-1,4-葡聚糖酶和β-1,4-葡萄糖苷酶同工酶多态性的研究。内切葡聚糖酶呈现出不同的电泳图谱。观察到三个活性区，即EG 1、EG 11和EG 111，显示出不同的电泳迁移率。一些分离株中观察到EG 1存在无效等位基因。在发育过程中，EG 1和EG 11始终存在，而EG 111在第八天出现。对于β-1,4-葡萄糖苷酶，观察到两个活性区，即β-葡糖1和β-葡糖11。β-葡糖1显示出可变的电泳迁移率。β-葡糖1在发育过程中始终存在，而β-葡糖11在第十二天出现。
