Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore117543.
Org Biomol Chem. 2010 Apr 21;8(8):1749-62. doi: 10.1039/b923331h.
Of the thousands of known chemical reactions, a handful of reactions, called "click" reactions, stand out with features such as good chemoselectivity, good solvent compatibilities, modularity, minimum synthetic demands, bioorthogonality and high yields. Among them, the Cu(i)-catalyzed 1,3-dipolar cycloaddition reaction between azides and terminal alkynes has emerged as a powerful tool in chemical biology and proteomics. This perspective surveys the significant contributions of click chemistry in catalomics (a sub-area in chemical proteomics), with special emphasis on activity-based protein profiling (ABPP), posttranslational modifications (PTMs) and enzyme inhibitor developments.
在数千种已知的化学反应中，有少数几种反应被称为“点击”反应，它们具有良好的化学选择性、良好的溶剂相容性、模块性、最小的合成要求、生物正交性和高收率等特点。其中，叠氮化物和末端炔烃之间的 Cu(i)催化 1,3-偶极环加成反应已成为化学生物学和蛋白质组学中的有力工具。本文综述了点击化学在催化组学（化学蛋白质组学的一个分支）中的重要贡献，特别强调了基于活性的蛋白质谱分析（ABPP）、翻译后修饰（PTMs）和酶抑制剂的发展。
