Marshall G E, Konstas A G, Lee W R
Department of Ophthalmology, University of Glasgow, United Kingdom.
Graefes Arch Clin Exp Ophthalmol. 1991;229(2):157-63. doi: 10.1007/BF00170550.
Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.
采用免疫金技术结合冷冻超薄切片术和伦敦白色树脂（LR white）包埋，我们研究了来自7只摘除的人眼（年龄范围63 - 78岁）角膜组织中I - IV型胶原蛋白和层粘连蛋白的精细结构分布。在任何角膜层中均未鉴定出II型胶原蛋白。I型和III型胶原蛋白以相似的方式分布在Bowman层和基质中的横纹胶原纤维中。IV型胶原蛋白仅位于Descemet膜的后部无条带区域。根据其公认的黏附功能，在角膜上皮下锚定斑和Descemet膜的内皮下区域鉴定出了层粘连蛋白。
