Ben-Zvi A, Rodrigues M M, Krachmer J H, Fujikawa L S
Curr Eye Res. 1986 Feb;5(2):105-17. doi: 10.3109/02713688609015099.
Collagen, fibronectin and laminin are important components of the extracellular matrix of the human cornea. We used the immunofluorescence technique with polyclonal antibodies directed against these proteins and to bullous pemphigoid antigen (BPA), in order to study their distribution in human corneas from 8 weeks of gestation to term and in adult corneas. Immunoreactivity was observed with antibodies to type I collagen in the limbus and the corneal stroma at 8 weeks of gestation. At 11 weeks of gestation it was found in epithelial basement membrane (EBM) and Descemet's membrane (DM) and continued thus throughout fetal and adult life. Type II collagen was not detected in fetal or adult cornea. Type III collagen was detected during 8-20th weeks of gestation in the EBM, DM and stroma. After 27th weeks of gestation, type III collagen could no longer be detected in the central cornea. Type IV collagen was detected in the EBM as early as 8 weeks of gestation and remained positive throughout fetal and adult life. Descemet's membrane was negative for type IV collagen at 8 weeks of gestation and became positive thereafter. Immunostaining for fibronectin in DM was negative at 8 weeks of gestation, followed by patchy staining of corneal stroma and EBM up to the age of 37 weeks of gestation. Staining in the EBM was negative or variable up to 70 years of age, and then became positive again in a 77 year old individual. Staining for LN was positive in the EBM after 8 weeks of gestation. Staining was negative in DM at that age, but became positive after 9 weeks of gestation. Staining for BPA was negative at 8-9 weeks of gestation, then gradually became positive.
胶原蛋白、纤连蛋白和层粘连蛋白是人类角膜细胞外基质的重要组成部分。我们使用针对这些蛋白质以及大疱性类天疱疮抗原（BPA）的多克隆抗体进行免疫荧光技术，以研究它们在从妊娠8周直至足月的人类角膜以及成人角膜中的分布情况。在妊娠8周时，在角膜缘和角膜基质中观察到针对I型胶原蛋白的抗体呈现免疫反应性。在妊娠11周时，它出现在上皮基底膜（EBM）和Descemet膜（DM）中，并在整个胎儿期和成年期持续存在。在胎儿或成人角膜中未检测到II型胶原蛋白。在妊娠8 - 20周期间，在EBM、DM和基质中检测到III型胶原蛋白。妊娠27周后，在中央角膜中无法再检测到III型胶原蛋白。早在妊娠8周时就在EBM中检测到IV型胶原蛋白，并且在整个胎儿期和成年期都保持阳性。在妊娠8周时，Descemet膜对IV型胶原蛋白呈阴性，此后变为阳性。在妊娠8周时，DM中纤连蛋白的免疫染色呈阴性，随后直至妊娠37周角膜基质和EBM出现斑片状染色。在70岁之前，EBM中的染色呈阴性或变化不定，然后在一名77岁个体中再次变为阳性。妊娠8周后，EBM中层粘连蛋白（LN）的染色呈阳性。在该年龄时，DM中的染色呈阴性，但在妊娠9周后变为阳性。在妊娠8 - 9周时，BPA染色呈阴性，然后逐渐变为阳性。
