Wu Hua-Sen, Gao Xiao-Ping, Xu Chang-Ping
Zhejiang Chinese Medical University, Hangzhou 310051, Zhejiang, China.
Zhongguo Yi Miao He Mian Yi. 2010 Feb;16(1):25-9.
A new TaqMan probe based real-time assay was developed to rapid detection of rubella virus.
The specific primer pair and probe were designed within the conserved P150 gene of rubella virus and the PCR reactive condition was optimized to improve the sensitivity and specificity of the assay. Clinical specimens collected from two outbreaks were detected by the developed assay.
The assay is specific to detect rubella virus. There is no cross-reactions to measles, mumps, influenza and other respiratory viruses. It could detect 0.01 TCID50/tube of rubella RNA and took only three hours to finish a detection. In addition, the assay is simple, accurate and repeatable.
The TaqMan based real-time PCR developed in this study provides a fast, sensitive and specific tool for molecular diagnosis on rubella virus.
开发一种基于TaqMan探针的实时检测方法，用于风疹病毒的快速检测。
在风疹病毒保守的P150基因内设计特异性引物对和探针，并优化PCR反应条件，以提高检测方法的灵敏度和特异性。采用所建立的检测方法对两次疫情采集的临床标本进行检测。
该检测方法对风疹病毒具有特异性，对麻疹、腮腺炎、流感及其他呼吸道病毒无交叉反应。能检测到0.01 TCID50/管的风疹RNA，完成一次检测仅需3小时。此外，该检测方法简便、准确、可重复。
本研究建立的基于TaqMan的实时PCR为风疹病毒的分子诊断提供了一种快速、灵敏、特异的工具。
