Ge Qiong, Yan Ju-Ying, Gong Li-Ming
ZheJiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, China.
Zhongguo Yi Miao He Mian Yi. 2010 Feb;16(1):38-43.
Study meningoencephalitis virus isolated from the Coxsackie B5 virus(CVB5 virus) VP1 gene characteristics of Zhejiang Province in 2008 and compare with other countries CVB5 prototype isolates, and to explore the relationship of variation of the Virus VP1 areas and epidemic viral meningoencephalitis
Hep-2 and RD cells in cerebrospinal fluid and stool specimens for virus isolation, positive isolates combination with intestinal type of serum. On the separation of virus extracted RNA, and then RT-PCR amplified VP, gene CVB5 virus fragments, and purification of sequencing products, using DNAMAN and Bioedit analytical processing software.
The VP1 gene of CVB5 isolated from Zhejiang province meningoencephalitis viral in 2008 was 735bp. There were no missing and insertion of nucleotide . Strains isolated from ZJ/12/02 with the nucleotide and amino acid sequence of the highest phylogenetic tree showed that they were not the same branch.
The mutation caused by viral meningoencephalitis virus CVB5 Zhejiang province in 2008 was smaller. Viral meningoencephalitis distributed in some areas in the province had no significant prevalence.
研究2008年浙江省从柯萨奇B5病毒（CVB5病毒）分离出的脑膜脑炎病毒VP1基因特征，并与其他国家CVB5原型毒株进行比较，探讨该病毒VP1区变异与病毒性脑膜脑炎流行的关系。
取脑脊液和粪便标本接种Hep-2和RD细胞进行病毒分离，阳性分离株与肠道型血清进行组合。对分离出的病毒提取RNA，然后用RT-PCR扩增CVB5病毒VP1基因片段，并对测序产物进行纯化，使用DNAMAN和Bioedit分析处理软件。
2008年浙江省从病毒性脑膜脑炎分离出的CVB5病毒VP1基因长度为735bp，无核苷酸缺失和插入。ZJ/12/02分离株与系统发育树中核苷酸和氨基酸序列最高的毒株不在同一分支。
2008年浙江省病毒性脑膜脑炎病毒CVB5变异较小，该病毒在省内部分地区分布，流行无明显规律。
