Gong Li-Ming, Ge Qiong, Yan Ju-Ying, Lu Yi-Yu, Feng Yan, Mao Hai-Yan, Li Min-Hong, Zhou Min
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310009, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Dec;26(12):971-4.
To study the gene characterization of enterovirus 71 (EV71) virus strains isolated from clinical specimens of children with hand-foot-and-mouth disease (HFMD) in Zhejiang province.
Virus were isolated from clinical samples including stool, throat swab and vesicle from patients with HFMD. The EV71 isolates were identified by microneutralization assay and reverse transcriptase PCR (RT-PCR) with specific primer pair for VP1 genes of EV71. Complete VP1 gene sequences (891 nucleotides) for recent 6 EV71 isolates were determined and compared with that of A, B, C genotype reference EV71 strains and 11 EV71 China isolates available from GeneBank by homogeneity and phylogenetic tree analyses.
9 strains of EV were isolated from 14 clinical specimens. Data from microneutralization and RT-PCR results indicated that all the strains belong to EV71. The nucleotide and amino acid homogeneity of these 6 Zhejiang strains with the representative isolates of A and B genotypes were 82.9%-85.5% and 94.9%-98.0% respectively; with the representative isolates of C were 89.2%-94.1% and 97.0%-99.0% respectively. There were 91.0%-92.2%, 90.2%-90.3%, 89.2%-89.5%, 96.7%-96.9% nucleotide, homology with representative strains of C1, C2, C3,C4 subgenotypes of EV71. The nucleotide homogeneity of these 6 EV71 isolated strains with 9 previously isolated Chinese strains appeared to be 93.8%-97.1%. These 6 EV71 isolated strains were within genotype C subgenogroup C4 in the phylogenetic tree.
The recently identified EV71 isolates in Zhejiang province belonged to subgenogroup C4.
研究从浙江省手足口病（HFMD）患儿临床标本中分离出的肠道病毒71型（EV71）毒株的基因特征。
从HFMD患者的粪便、咽拭子和水疱等临床样本中分离病毒。采用微量中和试验及针对EV71病毒VP1基因的特异性引物对进行逆转录聚合酶链反应（RT-PCR），对EV71分离株进行鉴定。测定了最近6株EV71分离株的完整VP1基因序列（891个核苷酸），并通过同源性分析和系统发育树分析，与A、B、C基因型的EV71参考毒株以及从基因库中获取的11株中国EV71分离株进行比较。
从14份临床标本中分离出9株EV。微量中和试验和RT-PCR结果表明，所有毒株均属于EV71。这6株浙江毒株与A、B基因型代表性分离株的核苷酸和氨基酸同源性分别为82.9% - 85.5%和94.9% - 98.0%；与C基因型代表性分离株的核苷酸和氨基酸同源性分别为89.2% - 94.1%和97.0% - 99.0%。与EV71的C1、C2、C3、C4亚基因型代表性毒株的核苷酸同源性分别为91.0% - 92.2%、90.2% - 90.3%、89.2% - 89.5%、96.7% - 96.9%。这6株EV71分离株与9株先前分离的中国毒株的核苷酸同源性为93.8% - 97.1%。在系统发育树中，这6株EV71分离株属于C基因型C4亚组。
浙江省最近鉴定出的EV71分离株属于C4亚组。
