人类胰腺辅脂酶基因定位于6号染色体短臂21.1区至末端。

Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter.

作者信息

Davis R C, Xia Y R, Mohandas T, Schotz M C, Lusis A J

机构信息

Lipid Research, VA Wadsworth Medical Center, Los Angeles, California 90073.

出版信息

Genomics. 1991 May;10(1):262-5. doi: 10.1016/0888-7543(91)90509-d.

DOI:10.1016/0888-7543(91)90509-d

PMID:2045105

Abstract

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Using primers derived from the known amino acid sequence, we have used the polymerase chain reaction to produce a cDNA clone corresponding to the complete coding region of the human procolipase mRNA. Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids indicated that the colipase gene (CLPS) resides on human chromosome 6. Further analysis of somatic cell hybrids carrying chromosome 6 translocations permitted regional localization of CLPS to the 6p21.1-pter region.

摘要

胰腺辅脂酶是一种12 kDa的多肽辅因子，作用于胰腺脂肪酶（EC 3.1.1.3），胰腺脂肪酶是膳食长链甘油三酯脂肪酸吸收所必需的一种酶。辅脂酶被认为能将脂肪酶非共价地锚定在脂质微团表面，抵消肠道胆汁盐的去稳定作用。利用从已知氨基酸序列推导的引物，我们通过聚合酶链反应产生了一个与人类前辅脂酶mRNA完整编码区相对应的cDNA克隆。对一组小鼠 - 人类体细胞杂种的基因组DNA进行Southern印迹分析表明，辅脂酶基因（CLPS）位于人类6号染色体上。对携带6号染色体易位的体细胞杂种进行进一步分析，可将CLPS定位到6p21.1 - pter区域。