Lowe M E
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
Gastroenterology. 1994 Nov;107(5):1524-36. doi: 10.1016/0016-5085(94)90559-2.
Dietary fats have an impact on health and disease. A pancreatic exocrine protein, pancreatic triglyceride lipase, is essential for the efficient digestion of dietary fats. This enzyme requires another pancreatic exocrine protein, colipase, for full activity in the gut lumen. In addition to its importance in fat digestion, pancreatic triglyceride lipase has potential applications in medical therapy, medical diagnostics, and industry. This potential stimulated interest in lipases; radiograph during the last few years, studies applying the technologies of molecular biology and radiograph crystallography greatly increased our knowledge about pancreatic triglyceride lipase and colipase protein structure, enzyme mechanism, and gene structure. This review focuses on these recent advances and discusses models for the kinetic properties of pancreatic triglyceride lipase and for the interaction of pancreatic triglyceride lipase with colipase.
膳食脂肪对健康和疾病有影响。一种胰腺外分泌蛋白，即胰腺甘油三酯脂肪酶，对于膳食脂肪的有效消化至关重要。这种酶在肠腔内充分发挥活性需要另一种胰腺外分泌蛋白，即辅脂酶。除了在脂肪消化中的重要性外，胰腺甘油三酯脂肪酶在医学治疗、医学诊断和工业领域也有潜在应用。这种潜力激发了人们对脂肪酶的兴趣；在过去几年中，应用分子生物学技术和X射线晶体学的研究极大地增加了我们对胰腺甘油三酯脂肪酶和辅脂酶的蛋白质结构、酶作用机制及基因结构的了解。本综述聚焦于这些最新进展，并讨论了胰腺甘油三酯脂肪酶的动力学特性模型以及胰腺甘油三酯脂肪酶与辅脂酶相互作用的模型。
