Department of Functional Biology, University of Valencia. Dr. Moliner, Spain.
Chemosphere. 2010 Jul;80(5):570-7. doi: 10.1016/j.chemosphere.2010.04.030. Epub 2010 May 6.
Gilthead sea bream Sparus aurata is the most commercialized Mediterranean aquacultured fish species. Ivermectin has recently (experimentally) started to be used to control ectoparasitic infestations in Mediterranean cultured marine fish. The potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35g) following oral administration at the recommended dose of 0.2 mgkg(-1) fish for 10d. Difference Gel Electrophoresis Technology (DIGE) was used to study the effect of this treatment in gilthead sea bream liver protein profile under routine culture conditions. The 2D-DIGE protein maps obtained were analyzed using the DeCyder 6.5 software. The results obtained showed significant changes in the expression of 36 proteins respect to the control group. Among these proteins, six increased in abundance, and 30 decreased. Spot showing differential expression respect to the control were analyzed by mass spectrometry and database search, which resulted in three positive identifications corresponding to hepatic proteins involved in lipid metabolism (apoA-I), oxidative stress responses and energy generation (beta-globin, ATP synthase subunit beta). These proteins have not been previously associated to invermectin effect.
金头鲷 Sparus aurata 是最商业化的地中海养殖鱼类。伊维菌素最近（实验性地）开始被用于控制地中海养殖海水鱼的外寄生虫感染。本研究在金头鲷幼鱼（35g）中，经口给予推荐剂量（0.2mg/kg 体重）伊维菌素 10d，研究其潜在的肝毒性。差异凝胶电泳技术（DIGE）用于研究在常规养殖条件下，该处理对金头鲷肝脏蛋白质图谱的影响。使用 DeCyder 6.5 软件分析获得的 2D-DIGE 蛋白质图谱。与对照组相比，结果显示 36 种蛋白质的表达有显著变化。其中，6 种蛋白表达增加，30 种蛋白表达减少。与对照组相比，差异表达的斑点通过质谱分析和数据库搜索进行分析，结果鉴定出三种与脂代谢（载脂蛋白 A-I）、氧化应激反应和能量生成（β-球蛋白、ATP 合酶亚基β）相关的肝蛋白。这些蛋白以前与伊维菌素的作用无关。
