Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, N-0310 Oslo, Norway.
Curr Biol. 2010 May 25;20(10):944-50. doi: 10.1016/j.cub.2010.03.068. Epub 2010 May 6.
Cytokinesis, the final step of cell division, conventionally proceeds to cell separation by abscission, or complete cytokinesis, but may in certain tissues be incomplete, yielding daughter cells that are interconnected in syncytia by stable intercellular bridges. The mechanisms that determine complete versus incomplete cytokinesis are not known. Here we report a novel in vivo role of the Drosophila CD2AP/CIN85 ortholog Cindr in both complete and incomplete cytokinesis. We also show evidence for the presence of persistent intercellular bridges in the major larval imaginal disc epithelia. During conventional division of both cultured and embryonic cells, Cindr localizes to cleavage furrows, intercellular bridges, and midbodies. Moreover, in cells undergoing incomplete cytokinesis in the female germline and the somatic ovarian follicle cell and larval imaginal disc epithelia, Cindr localizes to arrested cleavage furrows and stable intercellular bridges, respectively. In these structures, Cindr colocalizes with the essential cytokinesis regulator Anillin. We show that Cindr interacts with Anillin and that depletion of either Cindr or Anillin gives rise to binucleate cells and fewer intercellular bridges in vivo. We propose that Cindr and Anillin cooperate to promote intercellular bridge stability during incomplete cytokinesis in Drosophila melanogaster.
胞质分裂是细胞分裂的最后一步，通常通过胞质分离（即完全胞质分裂）进行细胞分离，但在某些组织中可能不完全，产生通过稳定的细胞间桥相互连接的合胞体子细胞。决定完全和不完全胞质分裂的机制尚不清楚。在这里，我们报告了果蝇 CD2AP/CIN85 同源物 Cindr 在完全和不完全胞质分裂中的新的体内作用。我们还证明了在主要的幼虫 imaginal 盘上皮细胞中存在持续的细胞间桥。在培养细胞和胚胎细胞的常规分裂过程中，Cindr 定位于分裂沟、细胞间桥和中体。此外，在雌性生殖系和体细胞卵巢滤泡细胞以及幼虫 imaginal 盘上皮细胞中进行不完全胞质分裂的细胞中，Cindr 分别定位于停滞的分裂沟和稳定的细胞间桥。在这些结构中，Cindr 与必需的胞质分裂调节剂 Anillin 共定位。我们表明 Cindr 与 Anillin 相互作用，并且 Cindr 或 Anillin 的耗竭都会导致体内双核细胞和更少的细胞间桥。我们提出，Cindr 和 Anillin 合作在果蝇不完全胞质分裂中促进细胞间桥的稳定性。
