STHdh(Q111)/Hdh(Q111) 细胞中的变化 microRNAs：miR-146a 靶向 TBP。
Altered microRNAs in STHdh(Q111)/Hdh(Q111) cells: miR-146a targets TBP.
机构信息
Structural Genomics Section, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Kolkata 700 064, India.
出版信息
Biochem Biophys Res Commun. 2010 Jun 4;396(3):742-7. doi: 10.1016/j.bbrc.2010.05.007. Epub 2010 May 6.
We studied expression of 90 miRNAs in STHdh(Q111)/Hdh(Q111) cells, a model for Huntington's disease and compared with that obtained in STHdh(Q7)/Hdh(Q7) cells. Fifteen miRNAs were down regulated and 12 miRNAs were up regulated more than 2-fold. Such changes were statistically significant. One hundred and forty-two genes are experimentally known targets of these altered miRNAs. It has been predicted that miR-146a may target Tata Binding Protein (TBP). Using luciferase reporter assays with 3'-UTRs of TBP, over-expression and inhibition of miR-146a, we showed that miR-146a targets TBP. Regulation of TBP by miR-146a may contribute to HD pathogenesis.
我们研究了亨廷顿病模型 STHdh(Q111)/Hdh(Q111)细胞中 90 种 miRNA 的表达情况，并与 STHdh(Q7)/Hdh(Q7)细胞中的表达情况进行了比较。有 15 种 miRNA 下调，12 种 miRNA 上调超过 2 倍。这些变化具有统计学意义。这些改变的 miRNA 的 142 个基因是实验已知的靶基因。已经预测 miR-146a 可能靶向 Tata 结合蛋白（TBP）。通过使用含有 TBP 3'-UTR 的荧光素酶报告基因检测、TBP 的过表达和 miR-146a 的抑制实验，我们表明 miR-146a 靶向 TBP。miR-146a 对 TBP 的调控可能有助于 HD 的发病机制。
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