Department of Inorganic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo, Japan.
Acta Biomater. 2010 Oct;6(10):4027-33. doi: 10.1016/j.actbio.2010.04.027. Epub 2010 May 6.
The ability of beta-tricalcium phosphate (beta-TCP) particles to store electric charge was confirmed by thermally stimulated depolarization current measurement as well as surface potential measurement. The efficacy of stored electrical charge on beta-TCP particles in enhancing nerve regeneration was evaluated. Bridge grafting was performed into sciatic nerve defects in Wistar rats with the following tubes: chitosan mesh tubes; chitosan mesh tubes on which beta-TCP particles with or without electrical polarization treatment had been immobilized (polarized and non-polarized tubes, respectively). As a control, isografts were used. Both motor and sensory nerve function as well as electrophysiological recovery progressed with time in each group. Immunofluorescence revealed rapider nerve regeneration in the polarized tube group compared with the non-polarized tube group. The axon density and axon area in the polarized tube group were significantly greater than those in the chitosan mesh tube and non-polarized group, and showed no significant differences from the control group. These results suggest that the stored charge on electrically polarized beta-TCP particles immobilized on chitosan mesh tubes may enhance nerve regeneration to the same extent as isografting.
β-磷酸三钙（β-TCP）颗粒储存电荷的能力通过热刺激去极化电流测量和表面电势测量得到了证实。评估了β-TCP 颗粒储存的电荷对促进神经再生的效果。将桥接移植物植入 Wistar 大鼠的坐骨神经缺损中，使用以下几种管：壳聚糖网管；壳聚糖网管上固定有β-TCP 颗粒，有或没有进行电极化处理（分别为极化管和非极化管）。作为对照，使用同种移植物。每组的运动和感觉神经功能以及电生理恢复都随着时间的推移而进展。免疫荧光显示，极化管组的神经再生速度快于非极化管组。极化管组的轴突密度和轴突面积明显大于壳聚糖网管和非极化组，与对照组无显著差异。这些结果表明，固定在壳聚糖网管上的电极化β-TCP 颗粒上储存的电荷可能会增强神经再生，其效果与同种移植物相同。
