Department of Inorganic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan.
J Biomed Mater Res A. 2009 Dec 15;91(4):994-1005. doi: 10.1002/jbm.a.32329.
We have constructed a chitosan nonwoven nanofiber mesh tube consisting of oriented fibers by the electrospinning method. The efficacy of oriented nanofibers on Schwann cell alignment and positive effect of this tube on peripheral nerve regeneration were confirmed. The physical properties of the chitosan nanofiber mesh sheets prepared by electrospinning with or without fiber orientation were characterized. Then, immortalized Schwann cells were cultured on these sheets. Furthermore, the chitosan nanofiber mesh tubes with or without orientation, and bilayered chitosan mesh tube with an inner layer of oriented nanofibers and an outer layer of randomized nanofibers were bridgegrafted into rat sciatic nerve defect. As a result of fiber orientation, the tensile strength along the axis of the sheet increased. Because Schwann cells aligned along the nanofibers, oriented fibrous sheets could exhibit a Schwann cell column. Functional recovery and electrophysiological recovery occurred in time in the oriented group as well as in the bilayered group, and approximately matched those in the isograft. Furthermore, histological analysis revealed that the sprouting of myelinated axons occurred vigorously followed by axonal maturation in the isograft, oriented, and bilayered group in the order. The oriented chitosan nanofiber mesh tube may be a promising substitute for autogenous nerve graft.
我们通过静电纺丝方法构建了一种由取向纤维组成的壳聚糖无纺纳米纤维网管。证实了取向纳米纤维对许旺细胞排列的功效，以及该管对周围神经再生的积极影响。通过静电纺丝制备的具有或不具有纤维取向的壳聚糖纳米纤维网片的物理性质进行了表征。然后，将永生化许旺细胞培养在这些片上。此外，将具有或不具有取向的壳聚糖纳米纤维网管以及具有内层取向纳米纤维和外层随机纳米纤维的双层壳聚糖网管桥接至大鼠坐骨神经缺损处。由于纤维取向，沿着片材的轴向的拉伸强度增加。由于许旺细胞沿着纳米纤维排列，因此取向纤维片可以表现出许旺细胞柱。在取向组和双层组中，功能恢复和电生理恢复及时发生，与同种异体移植相匹配。此外，组织学分析表明，在同种异体移植、取向和双层组中，有髓轴突的发芽强烈发生，随后是轴突成熟。取向壳聚糖纳米纤维网管可能是自体神经移植物的有前途的替代品。
