Hamburg University of Technology, Germany.
Acta Biomater. 2010 Oct;6(10):4127-35. doi: 10.1016/j.actbio.2010.04.015. Epub 2010 May 7.
Commonly, to determine osteoclastic resorption of biomaterials only the resorbed area is measured. The depth of the resorption pit, however, may also be important for the performance of a material. To generate such data we used two calcium phosphate ceramics (Ca(10) and Ca(2)). The solubility of the materials was determined according to DIN EN ISO 10993-14. They were scanned three-dimensionally using infinite focus microscopy and subsequently cultivated for 4 weeks in simulated body fluid without (control) or with human osteoclasts. After this cultivation period osteoclasts number was determined and surface changes were evaluated two- and three-dimensionally. Ca(10) and Ca(2) showed solubilities of 11.0+/-0.5 and 23.0+/-2.2 mgg(-1), respectively. Both materials induced a significant increase in osteoclast number. While Ca(10) did not show osteoclastic resorption, Ca(2) showed an increased pit area and pit volume due to osteoclastic action. This was caused by an increased average pit depth and an increased number of pits, while the average area of single pits did not change significantly. The deduced volumetric osteoclastic resorption rate (vORR) of Ca(2) (0.01-0.02 microm(3)microm(-2)day(-1)) was lower than the remodelling speed observed in vivo (0.08 microm(3)microm(-2)day(-1)), which is in line with the observation that implanted resorbable materials remain in the body longer than originally expected. Determination of volumetric indices of osteoclastic resorption might be valuable in obtaining additional information about cellular resorption of bone substitute materials. This may help facilitate the development of novel materials for bone substitution.
通常，为了确定生物材料的破骨细胞吸收，仅测量被吸收的区域。然而，吸收坑的深度对于材料的性能也可能很重要。为了生成此类数据，我们使用了两种磷酸钙陶瓷（Ca(10)和 Ca(2)）。根据 DIN EN ISO 10993-14 测定材料的溶解度。使用无限聚焦显微镜对它们进行三维扫描，然后在不含（对照）或含有人破骨细胞的模拟体液中培养 4 周。在此培养期后，确定破骨细胞数量，并从二维和三维评估表面变化。Ca(10)和 Ca(2)的溶解度分别为 11.0+/-0.5 和 23.0+/-2.2 mgg(-1)。这两种材料都导致破骨细胞数量显著增加。虽然 Ca(10)没有显示出破骨细胞吸收，但 Ca(2)由于破骨细胞的作用显示出增加的坑面积和坑体积。这是由于平均坑深度增加和坑数量增加所致，而单个坑的平均面积没有明显变化。推断的 Ca(2)的体积破骨细胞吸收率（vORR）（0.01-0.02 microm(3)microm(-2)day(-1)）低于体内观察到的重塑速度（0.08 microm(3)microm(-2)day(-1)），这与观察到的植入可吸收材料在体内停留的时间比预期的要长得多的观察结果一致。体积破骨细胞吸收指数的测定可能有助于获得有关骨替代材料细胞吸收的更多信息。这可能有助于促进新型骨替代材料的开发。
