Leonard J H, Kearsley J H, Chenevix-Trench G, Hayward N K
Queensland Institute of Medical Research, Herston, Australia.
Int J Cancer. 1991 Jun 19;48(4):511-5. doi: 10.1002/ijc.2910480406.
The presence of gene amplification was determined in 66 fresh head-and-neck SCC specimens using a battery of 9 different probes. Amplification of at least one gene was found in 12 samples (18%), of which 7 were amplified at multiple loci (58%). We observed amplifications for EGFR (10% of samples) and c-myc (9%), as well as co-amplification of bcl-1/int-2 (7%). No amplifications were demonstrated for c-Ha-ras-1, TGF alpha, c-mos, c-erbB-2, or c-erbA-2. The incidence of proto-oncogene amplification in head-and-neck SCC patients is comparable to that reported for other solid tumours. There was no statistically significant difference in survival between patients with or without gene amplification. However, the presence of multiple amplifications in several patients with advanced primary tumours suggests that the accumulation of genetic changes may correlate more closely with tumour size than with inherent biologic aggression.
使用一组9种不同的探针，对66份新鲜的头颈部鳞状细胞癌标本进行基因扩增检测。在12份样本（18%）中发现至少一种基因发生扩增，其中7份在多个位点发生扩增（58%）。我们观察到表皮生长因子受体（EGFR，10%的样本）和c-myc（9%）发生扩增，以及bcl-1/int-2共扩增（7%）。未检测到c-Ha-ras-1、转化生长因子α（TGF alpha）、c-mos、c-erbB-2或c-erbA-2发生扩增。头颈部鳞状细胞癌患者中原癌基因扩增的发生率与其他实体瘤报告的发生率相当。有或没有基因扩增的患者之间生存率无统计学显著差异。然而，在几例晚期原发性肿瘤患者中存在多个扩增，这表明基因变化的积累可能与肿瘤大小的相关性比与内在生物学侵袭性的相关性更密切。
