Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea.
Theriogenology. 2010 Aug;74(3):473-8. doi: 10.1016/j.theriogenology.2010.03.001. Epub 2010 May 8.
To determine factors that affect the efficiency of dog cloning by somatic cell nuclear transfer, the present study was performed to investigate 1) the effects of surgical history (non-operated/operated) and parity (nullipara/multipara) on the recovery of in vivo canine oocytes; 2) the effects of surgical history and parity of recipients on the pregnancy and delivery; and 3) the effects of synchronization state (AA, advanced asynchrony; SY, synchrony; RA, retarded asynchrony) between oocytes donor and recipient on the pregnancy and delivery. Oocyte recovery rate was significantly higher in non-operated dogs compared to operated dogs (93.8 vs. 89.6%, P < 0.05) and not different between nulliparous dogs and multiparous dogs. Delivery rate was also significantly higher in non-operated dogs compared to operated dogs (2.8 vs. 1.0%, P < 0.05) and in nulliparous dogs than multiparous dogs (3.0 vs. 1.7%, P < 0.05). Even though SY showed increased pregnancy and delivery rate (20.0% and 3.0%) compared to AA (15.0% and 2.0%) and RA (0.0% and 0.0%), there was no significant difference. In conclusion, we recommend non-operated dogs as experimental dogs and nulliparous dogs as recipient dogs to increase delivery rate after transfer of somatic cell nuclear transferred embryos, but further study is needed to find out appropriate synchrony status at the transfer.
为了确定影响体细胞核移植犬克隆效率的因素，本研究旨在调查：1）手术史（未手术/已手术）和产次（初产/经产）对犬体内卵母细胞回收的影响；2）受体的手术史和产次对妊娠和分娩的影响；3）卵母细胞供体和受体之间的同步状态（AA，提前不同步；SY，同步；RA，延迟不同步）对妊娠和分娩的影响。与手术犬相比，未手术犬的卵母细胞回收率显著更高（93.8%比 89.6%，P<0.05），而初产犬和经产犬之间无差异。与手术犬相比，未手术犬的分娩率也显著更高（2.8%比 1.0%，P<0.05），而初产犬比经产犬更高（3.0%比 1.7%，P<0.05）。尽管 SY 与 AA（15.0%和 2.0%）和 RA（0.0%和 0.0%）相比，妊娠和分娩率更高（20.0%和 3.0%），但无显著差异。总之，我们建议使用未手术犬作为实验犬，使用初产犬作为受体犬，以提高体细胞核移植胚胎移植后的分娩率，但需要进一步研究以确定合适的移植同步状态。
