Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, SE-58133 Linköping, Sweden.
J Pharm Biomed Anal. 2010 Nov 2;53(3):576-82. doi: 10.1016/j.jpba.2010.03.040. Epub 2010 Apr 10.
A validated, accurate and sensitive LC-MS/MS method for determination of olanzapine and its metabolite N-desmethylolanzapine has been developed. The analytes were quantified by tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring. Olanzapine and desmethylolanzapine were extracted from serum or cerebral spinal fluid samples, 200 microl, with tert-butyl methyl ether using olanzapine-D3 as internal standard. Calibrations for olanzapine and desmethylolanzapine were linear within the selected range of 0.2-30 ng/ml (6-96 nM) in cerebral spinal fluid and for olanzapine in plasma, in the range of 5-100 ng/ml (16-320 nM). The method was successfully used for the analysis of samples from patients treated with olanzapine in the dose range of 2.5-25mg/day.
已建立一种用于测定奥氮平和其代谢物 N-去甲奥氮平的验证、准确且灵敏的 LC-MS/MS 方法。采用正电喷雾电离模式和多重反应监测的串联质谱法对分析物进行定量分析。奥氮平和去甲奥氮平从血清或脑脊液样品中提取，使用叔丁基甲基醚，200μL，内标为奥氮平-D3。奥氮平和去甲奥氮平在脑脊液中的校准范围为 0.2-30ng/ml（6-96nM），奥氮平在血浆中的校准范围为 5-100ng/ml（16-320nM），均呈线性。该方法成功用于测定奥氮平剂量范围为 2.5-25mg/天的患者的样品分析。
