Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain.
J Pharm Biomed Anal. 2010 Nov 2;53(3):250-61. doi: 10.1016/j.jpba.2010.04.020. Epub 2010 Apr 20.
The applications of flow-through optosensing in clinical and pharmaceutical analysis in the last decade are here reviewed. A classification of the flow methodologies employed (conventional flow injection analysis, multicommutated flow analysis and sequential injection analysis) is introduced and their principal characteristics explained. The fundamentals of flow-through optosensing together with the main detection techniques are described and, later, the main applications of these flow methods are highlighted, paying special attention to the recent incorporation of new detection techniques in optosensing and to the design of multiparameter sensors. Finally, the advantages and disadvantages of the different flow methodologies here described are critically discussed.
本文回顾了过去十年中流动光学传感在临床和药物分析中的应用。介绍了所采用的流动方法（常规流动注射分析、多交替流动分析和顺序注射分析）的分类，并解释了它们的主要特点。描述了流动光学传感的基本原理以及主要的检测技术，然后重点介绍了这些流动方法的主要应用，特别关注了光学传感中新检测技术的引入和多参数传感器的设计。最后，批判性地讨论了本文所描述的不同流动方法的优缺点。
