Research Centre of Animal Production, Slovak Agricultural Research Centre, 949 92, Nitra, Slovakia.
Domest Anim Endocrinol. 2010 Jul;39(1):1-9. doi: 10.1016/j.domaniend.2009.06.001. Epub 2009 Jul 21.
The aim of our studies was to compare the roles of leptin and ghrelin in the direct control of proliferation, apoptosis, and secretory activity by porcine ovarian cells. In our in vitro experiments, we analyzed the effects of leptin and ghrelin treatments (at 0, 1, 10, or 100ng/mL medium) on the accumulation of proliferation-related peptides (PCNA, cyclin B1, MAP kinase [MAPK]) and apoptosis-associated peptides (Bax, caspase 3, p53), and on progesterone secretion by cultured porcine granulosa cells, using immunocytochemistry, SDS PAGE-Western immunoblotting, and radioimmunoassay (RIA). Leptin stimulated proliferation (PCNA, cyclin B1, MAPK), apoptosis (Bax, p53), and progesterone secretion. Ghrelin promoted proliferation (PCNA, cyclin B1, MAPK) and progesterone secretion but suppressed apoptosis (Bax, caspase 3, p53). These observations suggest that both leptin and ghrelin directly control proliferation, apoptosis, and secretory activity by porcine ovarian cells. At the level of the ovary, in contrast to the hypothalamo-hypophysial system, leptin and ghrelin may have similar action in promoting granulosa cell proliferation and progesterone secretion, but they may be antagonistic to one another (leptin, stimulator; ghrelin, inhibitor) in controlling apoptosis.
我们研究的目的是比较瘦素和胃饥饿素在直接控制猪卵巢细胞增殖、凋亡和分泌活性方面的作用。在我们的体外实验中，我们分析了瘦素和胃饥饿素处理（0、1、10 或 100ng/mL 培养基）对培养的猪颗粒细胞中增殖相关肽（PCNA、细胞周期蛋白 B1、MAP 激酶 [MAPK]）和凋亡相关肽（Bax、caspase 3、p53）积累以及孕激素分泌的影响，采用免疫细胞化学、SDS PAGE-Western 免疫印迹和放射免疫分析（RIA）。瘦素刺激增殖（PCNA、细胞周期蛋白 B1、MAPK）、凋亡（Bax、p53）和孕激素分泌。胃饥饿素促进增殖（PCNA、细胞周期蛋白 B1、MAPK）和孕激素分泌，但抑制凋亡（Bax、caspase 3、p53）。这些观察结果表明，瘦素和胃饥饿素均可直接控制猪卵巢细胞的增殖、凋亡和分泌活性。在卵巢水平上，与下丘脑-垂体系统相反，瘦素和胃饥饿素可能在促进颗粒细胞增殖和孕激素分泌方面具有相似的作用，但在控制凋亡方面可能相互拮抗（瘦素，刺激剂；胃饥饿素，抑制剂）。
