Department of Biology 371 Serra St.,Stanford University Stanford CA 94305-3020, USA.
Gene. 2010 Sep 1;463(1-2):18-20. doi: 10.1016/j.gene.2010.04.015. Epub 2010 May 7.
Recombination rate is a key evolutionary parameter that determines the degree to which sites are linked. Estimating recombination rates is thus of crucial importance for population genetic and molecular evolutionary studies. We present here a user-friendly web-based tool that can be used to retrieve recombination rate estimates for single and/or multiple loci in the Drosophila melanogaster genome given a user-defined choice of the genome release. We used the Marey map approach that is based on comparing the genetic and physical maps to infer recombination rates along the major chromosomes of the D.melanogaster genome. Our implementation of this approach is based on building third-order polynomials which are used to interpolate recombination rates at all points on the chromosome except for telomeric and centromeric regions in which such polynomials are known to provide particularly poor estimation.
重组率是决定位点连锁程度的关键进化参数。因此，估计重组率对于群体遗传和分子进化研究至关重要。我们在这里提供了一个用户友好的基于网络的工具，可以根据用户选择的基因组版本，为果蝇基因组中的单个或多个基因座检索重组率估计值。我们使用了 Marey 图谱方法，该方法基于比较遗传图谱和物理图谱，推断果蝇基因组主要染色体上的重组率。我们对这种方法的实现是基于构建三阶多项式，这些多项式用于在染色体上除端粒和着丝粒区域之外的所有点上进行重组率的内插，因为众所周知，在这些区域，这些多项式提供的估计特别差。
