Perinatology Research Branch, Intramural Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD/NIH/DHHS, Bethesda, MD, USA.
Am J Obstet Gynecol. 2010 May;202(5):462.e1-41. doi: 10.1016/j.ajog.2010.02.045.
The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture.
The transcriptome of amnion and chorion (n=20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction.
Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate<0.1; fold change>1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway.
The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.
本研究旨在比较胎膜破裂部位与远离破裂部位的羊膜和绒毛膜之间的转录组。
对自发胎膜早破且足月自然分娩的妇女的胎膜破裂部位和远离破裂部位的羊膜和绒毛膜（n=20 每个部位）的转录组进行 Illumina HumanHT-12 微阵列分析。使用定量逆转录聚合酶链反应验证了选定的基因。
在胎膜破裂和未破裂部位的绒毛膜之间有 677 个基因差异表达（假发现率<0.1；倍数变化>1.5）。定量逆转录聚合酶链反应在 14 个基因中的 10 个基因中证实了差异表达。富集的生物学过程包括解剖结构发育、细胞黏附和信号转导。细胞外基质-受体相互作用是受影响最大的信号通路。
足月产时自发胎膜早破后胎膜的转录组特征是参与特征途径的基因在区域和组织上的差异表达，其中包括细胞外基质-受体相互作用。
