微小RNA在人绒毛羊膜中随妊娠进展和炎症的差异表达。
Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes.
作者信息
Montenegro Daniel, Romero Roberto, Pineles Beth L, Tarca Adi L, Kim Yeon Mee, Draghici Sorin, Kusanovic Juan Pedro, Kim Jung-Sun, Erez Offer, Mazaki-Tovi Shali, Hassan Sonia, Espinoza Jimmy, Kim Chong Jai
机构信息
Perinatology Research Branch, National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services, Bethesda, MD, USA.
出版信息
Am J Obstet Gynecol. 2007 Sep;197(3):289.e1-6. doi: 10.1016/j.ajog.2007.06.027.
OBJECTIVE
The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation.
STUDY DESIGN
Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10).
RESULTS
More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories.
CONCLUSION
Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.
目的
本研究旨在确定随着孕周增加、临产及炎症反应，羊膜绒毛膜中微小RNA（miRNA）的差异表达情况。
研究设计
从以下几组患者获取羊膜绒毛膜中157种miRNA的表达谱：1）足月未临产（n = 10）；2）足月临产（n = 10）；3）早产合并组织学绒毛膜羊膜炎（n = 9）；4）无组织学绒毛膜羊膜炎（n = 10）。
结果
所筛选的miRNA中超过95%有表达。观察到13种miRNA的表达存在孕周依赖性变化。未临产女性与临产女性之间未观察到miRNA表达差异。有绒毛膜羊膜炎的胎膜中miR-223和miR-338表达增加。对差异表达miRNA靶向基因的基因本体分析显示特定生物学过程类别富集。
结论
随着孕周增加及绒毛膜羊膜炎发生，羊膜绒毛膜与一部分miRNA的差异表达有关。
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