Department of Zoology and Cell Biology, University of the Basque Country, Basque Country, Spain.
Mar Environ Res. 2010 Aug;70(2):142-9. doi: 10.1016/j.marenvres.2010.04.004. Epub 2010 Apr 24.
The Urdaibai estuary is a UNESCO biosphere reserve impacted by recreational, agricultural and industrial activities. Polycyclic aromatic hydrocarbons (PAHs) are major pollutants in Urdaibai, and their control and the identification of their sources is central on the preservation of the area. Chemical analysis by GC-MS showed a significant pollution by PAHs in Urdaibai sediment samples, mainly from pyrolytic sources, with minor contributions from oil spills. Measurement of the dioxin-like activity using a yeast-based bioassay showed an excess of biological activity in sediment samples from the inner part of the estuary. Analysis of PAH metabolites in bile of thicklip grey mullet (Chelon labrosus) showed a large excess of 2-naphthol over 1-naphthol or 1-pyrenol, suggesting a specific contamination in the inner Urdaibai estuary by some industrial process. Therefore, the combination of these three techniques defined different PAH pollution sources in Urdaibai: a major pyrolytic origin, occasional oil spills, and specific industrial activities.
乌达贝伊河口是一个受到休闲、农业和工业活动影响的联合国教科文组织生物圈保护区。多环芳烃（PAHs）是乌达贝伊的主要污染物，控制和识别其来源是保护该地区的核心。GC-MS 的化学分析表明，乌达贝伊沉积物样品受到多环芳烃的严重污染，主要来自热解源，石油泄漏的贡献较小。使用基于酵母的生物测定法测量二恶英样活性表明，河口内部的沉积物样品中存在过量的生物活性。厚唇灰鲻（Chelon labrosus）胆汁中多环芳烃代谢物的分析表明，2-萘酚相对于 1-萘酚或 1-苊醇过量，这表明乌达贝伊河口内部受到某种工业过程的特定污染。因此，这三种技术的结合确定了乌达贝伊的不同多环芳烃污染来源：主要的热解源、偶尔的石油泄漏和特定的工业活动。
