Diagnostics Department, Spedali Civili di Brescia, Brescia, Italy.
Clin Immunol. 2010 Aug;136(2):217-27. doi: 10.1016/j.clim.2010.04.005. Epub 2010 May 10.
A major problem in the field of stem cell transplantation is the difficulty to monitor the efficacy of immune reconstitution. By modifying the widely used method of measuring T-cell receptor excision circles (TRECs) and the recently proposed kappa-deleting recombination excision circles (KRECs) assay, we set up a duplex Real-Time PCR that allowed the simultaneous quantification of newly produced T and B cells in children with primary immunodeficiency undergone to transplantation. We found that lymphocyte recovery involves the mobilization of both new T and B cells from production and maturation sites, and that the increase of TRECs and KRECs can be or strictly associated or independent one from the other. Some patients showed a "lymphocyte rebound" which is followed by a progressive decrease of newly produced T and B lymphocytes starting about 2years after transplantation. In other patients, TRECs and KRECs number remained very low for the entire period of study.
干细胞移植领域的一个主要问题是难以监测免疫重建的效果。通过改进广泛使用的 T 细胞受体切除环（TRECs）测量方法和最近提出的κ缺失重组切除环（KRECs）检测方法，我们建立了一种双路实时 PCR，可同时定量原发性免疫缺陷接受移植的儿童中新产生的 T 和 B 细胞。我们发现淋巴细胞恢复涉及从产生和成熟部位动员新的 T 和 B 细胞，并且 TRECs 和 KRECs 的增加可以是相互关联的，也可以是相互独立的。一些患者出现“淋巴细胞反弹”，随后在移植后约 2 年开始新产生的 T 和 B 淋巴细胞逐渐减少。在其他患者中，TRECs 和 KRECs 的数量在整个研究期间都非常低。
