金黄色葡萄球菌中蛋白质细胞定位研究的荧光报告基因。
Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus.
机构信息
Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
出版信息
Appl Environ Microbiol. 2010 Jul;76(13):4346-53. doi: 10.1128/AEM.00359-10. Epub 2010 May 7.
Abstract
We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.
摘要
我们构建了一组质粒，可在金黄色葡萄球菌的天然染色体基因座上表达融合了四种不同荧光蛋白（绿色荧光蛋白[GFP]、青色荧光蛋白[CFP]、黄色荧光蛋白[YFP]和 mCherry）之一的蛋白质，使用了两种不同的抗性标记（卡那霉素和红霉素）。我们还构建了一个可在金黄色葡萄球菌染色体异位 spa 基因座上表达蛋白质的质粒。这个工具包可用于研究金黄色葡萄球菌中蛋白质的定位，金黄色葡萄球菌是一种在医疗保健和社区环境中都很突出的病原体。
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