用于在金黄色葡萄球菌中独立控制绿色和红色荧光融合蛋白表达的双质粒载体系统。
Two-plasmid vector system for independently controlled expression of green and red fluorescent fusion proteins in Staphylococcus aureus.
作者信息
Brzoska Anthony J, Firth Neville
机构信息
School of Biological Sciences, University of Sydney, Sydney, New South Wales, Australia.
出版信息
Appl Environ Microbiol. 2013 May;79(9):3133-6. doi: 10.1128/AEM.00144-13. Epub 2013 Mar 1.
Abstract
We have constructed a system for the regulated coexpression of green fluorescent protein (GFP) and red fluorescent protein (RFP) fusions in Staphylococcus aureus. It was validated by simultaneous localization of cell division proteins FtsZ and Noc and used to detect filament formation by an actin-like ParM plasmid partitioning protein in its native coccoid host.
摘要
我们构建了一个用于在金黄色葡萄球菌中调控绿色荧光蛋白（GFP）和红色荧光蛋白（RFP）融合蛋白共表达的系统。该系统通过细胞分裂蛋白FtsZ和Noc的同时定位得到验证，并用于检测其天然球菌宿主中肌动蛋白样ParM质粒分配蛋白的丝状形成。
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