Jones P D, Sadler T E, Castro J E
Dev Biol Stand. 1977;38:259-64.
The effects of i.v. C. parvum on the growth of a.s.c. inoculum of the Lewis lung carcinoma were studied in normal C57 B1 mice, and in those in which separate components of the immune response were impaired. C. parvum given either at the same time as tumour inoculation or when the carcinoma was 1 cm in diameter, and fully vascularised, reduced tumour growth. Macrophages were impaired by silica (Si), cortisone acetate (CA) or trypan blue (TB), and T cells by thymectomy and sublethal irradiation (TXR) or antilymphocyte serum (ALS). TB did not affect tumour size, whereas Si, CA, TXR, or ALS (initiated before tumour inoculation) reduced it. When C. parvum was given in combination with TB, or ALS (initiated after tumour inoculation), its antitumour effects were unchanged. C. parvum given after ALS (before tumour), TXR, Si, or CA had no further effect on tumour growth. We have shown that C. parvum inhibits the growth of inoculated Lewis tumour. However, it has not been possible to clearly define in vivo the immunological mechanisms involved.
在正常C57 B1小鼠以及免疫反应各单独成分受损的小鼠中，研究了静脉注射微小隐孢子虫（C. parvum）对Lewis肺癌腹水接种物生长的影响。在接种肿瘤的同时或当癌肿直径达到1厘米且完全血管化时给予微小隐孢子虫，可抑制肿瘤生长。巨噬细胞通过二氧化硅（Si）、醋酸可的松（CA）或台盼蓝（TB）受损，T细胞通过胸腺切除术和亚致死剂量照射（TXR）或抗淋巴细胞血清（ALS）受损。TB不影响肿瘤大小，而Si、CA、TXR或ALS（在肿瘤接种前开始使用）可使其减小。当微小隐孢子虫与TB或ALS（在肿瘤接种后开始使用）联合使用时，其抗肿瘤作用不变。在ALS（在肿瘤接种前）、TXR、Si或CA之后给予微小隐孢子虫，对肿瘤生长没有进一步影响。我们已经表明微小隐孢子虫可抑制接种的Lewis肿瘤的生长。然而，尚未能够在体内明确界定所涉及的免疫机制。
