Wu Yvonne T, Zhu Hua, Willcox Mark, Stapleton Fiona
Institute for Eye Research, University of New South Wales, Sydney, New South Wales, Australia.
Optom Vis Sci. 2010 Jul;87(7):465-8. doi: 10.1097/OPX.0b013e3181e172a1.
To determine the rate and type of microbial contamination when contact lens cases are air-dried in two different positions (face up and face down) and in four different locations (toilet, bathroom, office, and bedroom).
Unused contact lens cases (n = 97) were rinsed with 2-ml sterile phosphate buffered saline and then placed on facial tissue paper in different locations: humid (toilet and bathroom) and non-humid (office and bedroom) and air-dried at room temperature. After 24 h, the contact lens cases were collected and sampled for microbial numbers and microbial types identified using standard techniques. The microbial profile and the rate of contamination between different locations and positions were compared.
Irrespective of the air-drying location, contact lens cases positioned face up had a significantly higher contamination rate (34/48, 71%) compared with contact lens cases air-dried face down (6/49, 12%) (p < 0.001). For those contact lens cases air-dried face up, there was more contamination when placed in humid environments (toilet and bathroom) than in the non-humid environments (office and bedroom) (p = 0.01). However, the contact lens case contamination rate among various locations was similar when contact lens cases were air-dried face down. Total microorganisms recovered from contact lens cases ranged from 0 to 275 colony forming unit per well. The most frequently recovered microorganisms from the contaminated contact lens cases were coagulase-negative Staphylococci, fungi, and Bacillus spp. Thirty-three percent (13/40) of contact lens cases were contaminated with multiple species.
Small numbers of microorganisms from the environment may contaminate contact lens cases while cases are air-dried face up. Cases air-dried in humid environments have higher levels of microbial contamination; this is particularly true when contact lens cases are positioned face up. On the basis of this limited study, we would recommend contact lens cases be air-dried face down.
确定隐形眼镜盒在两种不同放置位置（面朝上和面朝下）以及四个不同地点（厕所、浴室、办公室和卧室）进行风干时的微生物污染率及污染类型。
将97个未使用过的隐形眼镜盒用2毫升无菌磷酸盐缓冲盐水冲洗，然后放置在不同地点的面巾纸上：潮湿环境（厕所和浴室）和非潮湿环境（办公室和卧室），并在室温下进行风干。24小时后，收集隐形眼镜盒并进行采样，以确定微生物数量和通过标准技术鉴定微生物类型。比较不同地点和位置之间的微生物分布情况及污染率。
无论风干地点如何，面朝上放置的隐形眼镜盒污染率（34/48，71%）显著高于面朝下风干的隐形眼镜盒（6/49，12%）（p<0.001）。对于面朝上风干的隐形眼镜盒，放置在潮湿环境（厕所和浴室）中的污染情况比在非潮湿环境（办公室和卧室）中更严重（p = 0.01）。然而，当隐形眼镜盒面朝下风干时，不同地点之间的污染率相似。从隐形眼镜盒中回收的总微生物数量范围为每孔0至275个菌落形成单位。受污染的隐形眼镜盒中最常回收的微生物是凝固酶阴性葡萄球菌、真菌和芽孢杆菌属。33%（13/40）的隐形眼镜盒被多种微生物污染。
在隐形眼镜盒面朝上风干时，环境中的少量微生物可能会对其造成污染。在潮湿环境中风干的隐形眼镜盒微生物污染水平更高；当隐形眼镜盒面朝上放置时尤其如此。基于这项有限的研究，我们建议将隐形眼镜盒面朝下风干。
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