Amos Christopher F, George Melanie D
CIBA Vision Corporation, 11460 Johns Creek Pky, Duluth, GA 30097, USA.
Cont Lens Anterior Eye. 2006 Dec;29(5):247-55. doi: 10.1016/j.clae.2006.09.007. Epub 2006 Nov 2.
Lens case contamination is a well-documented occurrence for contact lens wearers despite the efficacy of current lens disinfectants. Several microorganisms have a propensity to attach to surfaces and may become more tolerant of disinfection upon attachment. Non-compliance with disinfection regimens occasionally occurs wherein patients store their lenses in saline or in tap water. A silver-impregnated lens case was developed in an attempt to decrease case contamination. These studies examine the performance of the case both in vitro and in vivo.
In vitro, lens cases were challenged with 10(3) microorganism suspensions of bacteria. After 24h incubation at room temperature, aliquots of inocula were removed and spread plated onto appropriate growth media. Surviving colonies were counted and microorganism log drop values from initial challenge inocula were determined. Two 40-subject 1-month contralateral clinical studies were conducted with SOLO-care Aqua solution using one bowl of a silver-impregnated case and one bowl of a standard lens case for lens storage. Lens cases were collected and cultured for aerobic bacteria.
In vitro efficacy data show significantly lower numbers of recovered microbes from silver-impregnated cases than from control cases. In both clinical studies, silver-impregnated cases had a statistically significantly lower proportion of bacterial contamination than control cases. The majority of microorganisms isolated from silver-impregnated cases were members of the normal skin flora.
The performance of the silver-impregnated case in vitro and the observed lower proportion of contaminated silver-impregnated lens cases in a clinical setting demonstrate the case's ability to decrease bacterial contamination.
尽管目前的镜片消毒剂有效，但对于隐形眼镜佩戴者而言，镜盒污染是有充分文献记载的现象。几种微生物易于附着在表面，并且附着后可能对消毒更具耐受性。患者偶尔会不遵守消毒方案，将镜片储存在盐水中或自来水中。为了减少镜盒污染，研发了一种含银镜盒。这些研究在体外和体内检验了该镜盒的性能。
在体外，用10³个细菌微生物悬液对镜盒进行挑战。在室温下孵育24小时后，取出接种物的等分试样，涂布在合适的生长培养基上。对存活菌落进行计数，并确定相对于初始挑战接种物的微生物对数下降值。使用SOLO-care Aqua溶液进行了两项有40名受试者参与的为期1个月的对侧临床研究，将一个含银镜盒的碗和一个标准镜片储存镜盒的碗用于镜片储存。收集镜盒并培养需氧菌。
体外效能数据显示，从含银镜盒中回收的微生物数量明显低于对照镜盒。在两项临床研究中，含银镜盒的细菌污染比例在统计学上均显著低于对照镜盒。从含银镜盒中分离出的大多数微生物是正常皮肤菌群的成员。
含银镜盒在体外的性能以及在临床环境中观察到的较低污染比例表明该镜盒能够减少细菌污染。
