Brien Holden Vision Institute, Sydney, Australia.
Eye Contact Lens. 2011 Nov;37(6):365-9. doi: 10.1097/ICL.0b013e318233c901.
Lens storage case hygiene practices are important for safe contact lens wear. However, the effectiveness of the manufacturer's direction for use and various cleaning regimens in reducing biofilm load is yet to be evaluated and compared. This in vitro study compared the effectiveness of several cleaning methods using silver-impregnated lens case and hydrogen peroxide disinfection systems.
Biofilms of Pseudomonas aeruginosa 122 and Staphylococcus aureus ATCC 6538 were grown in silver-impregnated and hydrogen peroxide lens cases. After the establishment of the biofilms, the silver-impregnated case was subjected to one of four cleaning regimens: "manufacturer's directions for use--rinsed and recapped"; "rubbed, rinsed, and recapped"; "rubbed, rinsed, and air dried"; or "rubbed, rinsed, tissue wiped, and air dried." Hydrogen peroxide cases underwent one of two regimens: "manufacturer's directions for use--rinsed with saline and air dried" or "soaked in solution for 6 hrs." The level of residual bacteria was quantified. The efficacy of each cleaning regimen was then compared.
Mechanical rubbing and wiping of silver-impregnated cases and soaking hydrogen peroxide cases in hydrogen peroxide were the most effective treatments for reducing biofilms. Once the silver-impregnated cases were rubbed, air drying or recapping the cases did not have any significant effect on the level of the biofilm. The level of initial biofilm formation of silver-impregnated cases was significantly lower than those of polypropylene lens cases (P<0.0001).
Rubbing and rinsing with disinfecting solution and wiping with a tissue can be considered to be effective in removing biofilms from silver-impregnated lens cases. Resoaking the basket-type lens case in hydrogen peroxide between use was found to be effective in removing biofilms from these cases.
镜片储存盒的卫生操作对于安全佩戴隐形眼镜至关重要。然而，制造商的使用说明以及各种清洁方案在减少生物膜负荷方面的有效性仍有待评估和比较。本体外研究比较了几种清洁方法在使用含银镜片盒和过氧化氢消毒系统时的效果。
在含银和过氧化氢镜片盒中培养铜绿假单胞菌 122 和金黄色葡萄球菌 ATCC 6538 的生物膜。生物膜形成后，对含银镜片盒进行以下四种清洁方案之一的处理：“按制造商说明——冲洗并盖上盖子”；“揉搓、冲洗并盖上盖子”；“揉搓、冲洗并风干”；或“揉搓、冲洗、用纸巾擦干并风干”。过氧化氢镜片盒采用以下两种方案之一进行处理：“按制造商说明——用生理盐水冲洗并风干”或“浸泡在溶液中 6 小时”。定量残留细菌的数量。然后比较每种清洁方案的效果。
机械揉搓和擦拭含银镜片盒以及将过氧化氢镜片盒浸泡在过氧化氢中是减少生物膜最有效的方法。一旦对含银镜片盒进行了揉搓，风干或盖上盖子并不会对生物膜的水平产生显著影响。含银镜片盒初始生物膜形成的水平明显低于聚丙烯镜片盒（P<0.0001）。
揉搓、用消毒剂冲洗和用纸巾擦拭可被认为是从含银镜片盒中有效去除生物膜的方法。在使用之间将篮式镜片盒重新浸泡在过氧化氢中被发现可以有效去除这些镜片盒中的生物膜。
