Institute of Ecology, Taizhou University, Linhai City, Zheijang Province, PR China.
Can J Microbiol. 2010 Apr;56(4):352-5. doi: 10.1139/w10-009.
To explore changes in the structure and diversity of activated sludge-derived microbial communities during adaptation to gradual increases in the concentration of wastewater, RAPD-PCR and the combination of PCR amplification of 16S rRNA genes with denaturing gradient gel electrophoresis (DGGE) analysis were used. In bacterial communities exposed to 0%, 5%, 10%, 20%, or 40% wastewater, there were 27, 25, 18, 17 and 16 bands, respectively, based on DGGE data, while there were 69, 83, 97, 86, and 88 bands, respectively, based on RAPD data. The community similarity index among bacterial communities during the process of adaptation to different concentrations of wastewater was different based on DGGE and RAPD data. Based on DGGE and RAPD profiles, the Shannon-Weiner and Simpson's diversity indices decreased sharply upon exposure to 10% wastewater, indicating that 10% wastewater might be a critical point at which the growth of bacteria could be significantly inhibited and the genotypic diversity could change. This indicated that changes in structure and diversity might have an inhibitory effect on the toxicity of organic matter and that selection and adaptation could play important roles in the changes.
为了探索在适应废水浓度逐渐增加的过程中，活性污泥衍生微生物群落结构和多样性的变化，我们采用了 RAPD-PCR 技术和聚合酶链反应扩增 16S rRNA 基因与变性梯度凝胶电泳（DGGE）分析相结合的方法。在暴露于 0%、5%、10%、20%或 40%废水中的细菌群落中，DGGE 数据分别有 27、25、18、17 和 16 条带，而 RAPD 数据分别有 69、83、97、86 和 88 条带。基于 DGGE 和 RAPD 数据，适应不同浓度废水过程中细菌群落之间的群落相似性指数不同。基于 DGGE 和 RAPD 图谱，当暴露于 10%废水时，香农-威纳和辛普森多样性指数急剧下降，表明 10%废水可能是细菌生长受到显著抑制且基因型多样性发生变化的临界点。这表明结构和多样性的变化可能对有机物的毒性有抑制作用，选择和适应可能在这些变化中发挥重要作用。
