Institute of Ecology, Taizhou University, 605 Dongfang Dadao, Linhai 317000, PR China.
J Hazard Mater. 2009 Dec 15;172(1):432-8. doi: 10.1016/j.jhazmat.2009.07.031. Epub 2009 Jul 15.
In order to explore the effect of hypersaline aniline-containing pharmaceutical wastewater on the structure of activated sludge-derived bacterial community, RAPD-PCR and PCR-DGGE techniques were used to analyze the change of bacterial community diversity and structure during the acclimation to different concentrations of two types of wastewater. Different bacterial community structures and significant shift in the bacterial community diversity were observed during the acclimation of wastewater. The number of bacterial species and the abundance of bacteria acclimated to different concentration of wastewater decreased when the concentration increased. The trends of bacterial community diversity indices revealed by RAPD or DGGE profiles were different. The change of bacterial community diversity might be due to the adaptation and mutation under selection issues in the acclimation of wastewater. The relationship between diversity indices and the content of chemical oxygen demand (COD), chloride, salinity and aniline based on RAPD and DGGE data indicated that the change of the bacterial community diversity might not be due to a single factor but might be due to the integrated effects of all the contaminants in the wastewater. Phylogenic trees based on RAPD and DGGE fingerprints were different, indicating that the effect of wastewaters A and B on the bacterial community structure was different, which might be due to the characteristics of wastewater.
为了探讨高盐苯胺类制药废水对活性污泥菌胶团细菌群落结构的影响，采用 RAPD-PCR 和 PCR-DGGE 技术分析了在驯化过程中两种废水不同浓度下细菌群落多样性和结构的变化。在废水驯化过程中观察到不同的细菌群落结构和细菌群落多样性的显著变化。随着浓度的增加，驯化到不同浓度废水中的细菌种类数量和细菌丰度减少。RAPD 或 DGGE 图谱揭示的细菌群落多样性趋势不同。细菌群落多样性的变化可能是由于废水驯化过程中的选择问题下的适应和突变。基于 RAPD 和 DGGE 数据的多样性指数与化学需氧量（COD）、氯离子、盐度和苯胺含量之间的关系表明，细菌群落多样性的变化可能不是由于单一因素引起的，而可能是由于废水中所有污染物的综合影响所致。基于 RAPD 和 DGGE 指纹图谱的系统发育树不同，表明废水 A 和 B 对细菌群落结构的影响不同，这可能是由于废水的特性所致。
